pancreatic duct epithelial cell line h6c7 hpde  (Absolute Biotech Inc)

Journal: Molecular Therapy Oncology

Article Title: Enhancing cancer killing and natural killer cell persistence by targeting NOXA, a predictor of poor patient survival

doi: 10.1016/j.omton.2026.201152

Figure Lengend Snippet: Cancer-derived conditioned media induces apoptosis-related gene expression and dysfunction in NK cells Human primary NK cells were incubated with control media (RPMI), AsPC-1-CM, or Capan-2-CM, followed by functional and molecular analyses. (A) NK cells were treated with CM for 72 h and stained with Annexin V/7-aminoactinomycin D (7-AAD) to assess apoptosis. (B) Metabolic activity in NK cells after 24 h of CM exposure was measured by oxygen consumption rate (OCR) and extracellular acidification rate (ECAR). (C) CM-pretreated NK cells were co-cultured with AsPC-1-Luc or Capan-2-Luc cancer cells for 24 h, and cancer-cell-killing activity was measured. (D) Expression of apoptosis-related proteins BIM and NOXA in CM-treated NK cells was analyzed using western blotting. Representative blots show NOXA and BIM levels following 24 h exposure to CM derived from normal pancreatic epithelial cells (HPDE) or pancreatic cancer cell lines (AsPC-1 and Capan-2). BIM and NOXA protein levels were quantified from three independent experiments and normalized to β-actin. (E) Western blots showing the expression of NOXA and BIM in NK cells following 20 h exposure to IL-6, IL-10, or TGF-β1. All data are presented as mean ± standard error of the mean (SEM). p values were calculated using one-way ANOVA with multiple comparison tests (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001).

Article Snippet: The human pancreatic duct epithelial cell line (H6c7) (HPDE) was purchased from Kerafast (Boston, MA, USA) and cultured using Keratinocyte SFM (1X) kit (#17005042; Thermo Fisher Scientific, Waltham, MA, USA) with 1% AA solution according to the manufacturer’s growth conditions and subculturing method.

Techniques: Derivative Assay, Gene Expression, Incubation, Control, Functional Assay, Staining, Activity Assay, Cell Culture, Expressing, Western Blot, Comparison

Journal: bioRxiv

Article Title: Human decellularized extracellular matrix from adipose tissue is a permissive microenvironment for pancreatic organoids generation

doi: 10.64898/2026.03.12.711286

Figure Lengend Snippet: (A) H6C7 derived pancreatic organoids in atdECM coating or (B) Matrigel coating stained with Coll IV, Cytokeratin 14, Laminin α5, E-cadherin, Giantin or Ki67. Scale bars: 100 µm. (C) Ki67 signal quantification normalized to DAPI surface staining according to the coating (n=2 donors).

Article Snippet: Pancreatic H6c7 cells, derived from normal and benign adult human pancreatic tissue via infection with human papillomavirus 16 [ ], were purchased from Kerafast company (ECA001-FP) (Kerafast, Boston, USA).

Techniques: Derivative Assay, Staining

Journal: Annals of Medicine and Surgery

Article Title: Combination of cycling hyperthermia and echinacoside creates a synergistic curing effect on pancreatic cancer PANC-1 cells

doi: 10.1097/MS9.0000000000003545

Figure Lengend Snippet: Effect of Ech alone or in combination with TC-HT on the cell viability. (A) MTT viability assay of PANC-1 cells treated with different concentrations of Ech or in combination with TC-HT treatment. (B) MTT viability assay of H6c7 normal human pancreatic cells treated with 20 μM Ech or in combination with the same TC-HT treatment. Data represent the mean ± standard deviation ( n = 3). Statistical significance was determined by one-way ANOVA followed by Tukey’s post hoc test (*** P < 0.001).

Article Snippet: H6c7 human pancreatic duct epithelial cell line was obtained from Kerafast, Inc. (Absolute Biotech, Boston, MA, USA) and maintained in keratinocyte serum-free medium (Invitrogen; Thermo Fisher Scientific, Inc., Carlsbad, CA, USA) supplemented with human recombinant epidermal growth factor, bovine pituitary extract (Invitrogen; Thermo Fisher Scientific, Inc.), and 1% (v/v) penicillin and streptomycin.

Techniques: MTT Viability Assay, Standard Deviation